Ethidium bromide binding to polyriboguanylic acid
نویسندگان
چکیده
منابع مشابه
Localization of the major ethidium bromide binding site on tRNA.
Binding of ethidium bromide to Escherichia coli tRNAVal and an RNA minihelix based on the acceptor stem and T-arm of tRNAVal was investigated by 19F and 1H NMR spectroscopy of RNAs labeled with fluorine by incorporation of 5-fluorouracil. Ethidium bromide selectively intercalates into the acceptor stem of the tRNAVal. More than one ethidium bromide binding site is found in the acceptor stem, th...
متن کاملBinding of ethidium bromide to a DNA triple helix. Evidence for intercalation.
The interaction of ethidium, a DNA intercalator, with the poly(dA).poly(dT) duplex and the poly (dA).2poly(dT) triplex has been investigated by a variety of spectrophotometric and hydrodynamic techniques. The fluorescence of ethidium is increased when either the duplex or triplex form is present. Binding constants, determined from absorbance measurements, indicate that binding to the triple hel...
متن کاملCircular dichroism studies of ethidium bromide binding to the isolated nucleolus.
Circular dichroism in the 300-360 nm region and fluorescence induced by intercaltating binding of ethidum bromide to both DNA and RNA components were studied in isolated HeLa nucleoli. Both DNA and RNA compoents contribute to the induced dichroic elliticity. Digestion of nucleoli by RNase or DNase shows that most of the induced ellipticity comes from the DNA component. In nucleoli with an RNA/D...
متن کاملBinding of polylysine and ethidium bromide to nucleosomal DNA: comparison of biochemical and electron microscopical results.
Ethidium bromide and polylysine interact with nucleosomal DNA and lead to change of biochemical properties and to morphological changes as to the distance between the two core particles of a nucleosome dimer. With increasing polylysine concentration, the buoyant density of nucleosomes decrease and the accessibility of the nucleosomal DNA to micrococcal nuclease is lowered. Electron microscopy o...
متن کاملMechanism of Ethidium Bromide Fluorescence Enhancement on
The mechanism of the enhancement of the fluorescence of ethidium bromide on binding to double helical RNA and DNA has been investigated. From an examination of the effect of different solvents on the fluorescence lifetime, quenching of fluorescence by proton acceptors, and the substantial lengthening of lifetime observed upon deuteration of the amino protons, regardless of the medium, we conclu...
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ژورنال
عنوان ژورنال: Biopolymers and Cell
سال: 1989
ISSN: 0233-7657,1993-6842
DOI: 10.7124/bc.0000c6